ATP-bioluminescence technique for fruit quality assessments: a detailed methodology for analysis of adenylate nucleotides
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SaquetA. A. (2017). ATP-bioluminescence technique for fruit quality assessments: a detailed methodology for analysis of adenylate nucleotides. Boletim Técnico-Científico, 3(1). https://doi.org/10.26669/2359-2664.2017.149

Resumo

The bioluminescence technique has been used during a long time for measurement
of adenylate nucleotides (ATP, ADP, and AMP) in several investigation fields.
Until the end of the seventies, the assay was complicated and the results uncertain
because of the low purity reagents, which were initially, extracted directly from
desiccated lanterns of firefly (Photinus pyralis), and poorly controlled reaction
conditions at the laboratory. With the introduction of a new type of luciferinluciferase
reagent, the ATP Monitoring Reagent (AMR), these disadvantages have
been overcome, and therefore, the application of the assay has been extended.
The new reagent results in the emission of light of an almost constant intensity,
proportional to the ATP concentration. This is in contrast to the flash obtained
with conventional reagents. This new technique facilitates the measurements and
improves the sensitivity of the assay. The reagent may also be used to monitor
changes in ATP concentration because the ratio between the ATP concentration and
light intensity remains constant. This work describes the principle and a detailed
methodology for determination of ATP, ADP, and AMP by the luciferin-luciferase
reaction in fruits such as apples, pears and sweet cherries and some applications.
https://doi.org/10.26669/2359-2664.2017.149
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Referências

ALMEIDA, D.; SAQUET, A. A. Mapping the gradients within Rocha pear fruit. In: INTERNATIONAL

POSTHARVEST SYMPOSIUM, 8., 2016, Cartagena, Spain. Anais… Cartagena: Programme

and Book of Abstracts, 2016. p.117.

GEIGENBERGER, P.; RIEWE, D.; FERNIE, A.R. The central regulation of plant physiology by

adenylates. Trends in Plant Science, v.15, n.2, p.98-105, 2009.

GOULD, S.J.; SUBRAMANI, S. Firefly luciferase as a tool in molecular and cell biology. Analytical

Biochemistry, v.175, p.5–13, 1988.

HARB, J.; STREIF, J.; SAQUET, A.A. Impact of controlled atmosphere storage conditions on storability

and consumer acceptability of ‘Regina’ sweet cherries. Journal of Horticultural Science

& Biotechnology, v.78, n.4, p.574-579, 2003.

HARVEY, E.N. Bioluminescence. Academic Press: New York, 1952.

LUNDIN, A. Bioluminescence and chemiluminescence - Part C. Methods in Enzymology, v.305,

p.346-346, 2000.

MAZLIAK, P. Desaturation processes in fatty acid and acyl lipid biosynthesis. Journal of Plant

Physiology, v.143, p.399-406, 1994.

MISKA, W.; GEIGER, R. A new type of ultrasensitive bioluminogenic enzyme substrates. I. Enzyme

substrates with D-luciferin as leaving group. Biological Chemistry, v.369, p.407–411, 1988.

OHLROGGE, J.; BROWSE, J. Lipid synthesis. The Plant Cell, v.7, p.957-970, 1995.

RONAGHI, M. et al. Real-time DNA sequencing using detection of pyrophosphate release. Analytical

Biochemistry, v.242, p.84–89, 1996.

SAQUET, A. A.; STREIF, J.; BANGERTH, F. Changes in ATP, ADP and pyridine nucleotide levels

related to the incidence of physiological disorders in ‘Conference’ pears and ‘Jonagold’ apples

during controlled atmosphere storage. Journal of Horticultural Science & Biotechnology, v.75,

n.2, p.243-249, 2000.

SAQUET, A. A.; STREIF, J.; BANGERTH, F. On the involvement of adenine nucleotides in the

development of brown heart in ‘Conference’ pears during delayed controlled atmosphere storage.

Gartenbauwissenschaft, v.66, n.3, p.140-144, 2001.

SAQUET, A. A. Untersuchungen zur Entstehung physiologischer Fruchterkrankungen sowie zur

mangelhaften Aromabildung von Conference Birnen und Jonagold Äpfeln unter verschiedenen

CA-Lagerbedingungen. 2001. 191 f. Tese (Doutorado). Universität Hohenheim. Stuttgart, 2001.

SAQUET, A. A.; STREIF, J.; BANGERTH, F. Impaired aroma production of CA-stored ‘Jonagold’ apples as

affected by adenine and pyridine nucleotide levels and fatty acid concentrations. Journal of Horticultural

Science & Biotechnology, v.78, n.5, p.695-705, 2003a.

SAQUET, A. A.; STREIF, J.; BANGERTH, F. Reducing internal browning disorders in ‘Braeburn’ apples by

delayed controlled atmosphere storage and some related physiological and biochemical changes. Acta Horticulturae

(ISHS), n.628, p.453-458, 2003b.

SAQUET, A. A.; ALMEIDA, D. Adenylate nucleotides and energy charge in ‘Rocha’ pear. In: ANNUAL

CONFERENCE OF THE COST ACTION FA, 4., 2015, Lisboa. Anais… Lisboa, 2015.

STREIF, J.; SAQUET, A. A. Internal flesh browning of Elstar apples as influenced by pre- and postharvest

factors. Acta Horticulturae (ISHS), n.599, p.523-527. 2003.

TAN, T. Stoffwechselphysiologische Untersuchungen zur Ursache der verminderten Aromabildung

von früh geernteten Apfel- und Erdbeerfrüchten. 1999. 188 f. Tese (Doutorado). Universität Hohenheim.

Stuttgart, 1999.

TAN, T.; BANGERTH, F. Are adenine and/or pyridine nucleotides involved in the volatile production of

prematurely harvested or long-term ULO stored apple fruits? Acta Horticulturae, n.553, p.215-218, 2001.