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Resumo
The bioluminescence technique has been used during a long time for measurementof adenylate nucleotides (ATP, ADP, and AMP) in several investigation fields.
Until the end of the seventies, the assay was complicated and the results uncertain
because of the low purity reagents, which were initially, extracted directly from
desiccated lanterns of firefly (Photinus pyralis), and poorly controlled reaction
conditions at the laboratory. With the introduction of a new type of luciferinluciferase
reagent, the ATP Monitoring Reagent (AMR), these disadvantages have
been overcome, and therefore, the application of the assay has been extended.
The new reagent results in the emission of light of an almost constant intensity,
proportional to the ATP concentration. This is in contrast to the flash obtained
with conventional reagents. This new technique facilitates the measurements and
improves the sensitivity of the assay. The reagent may also be used to monitor
changes in ATP concentration because the ratio between the ATP concentration and
light intensity remains constant. This work describes the principle and a detailed
methodology for determination of ATP, ADP, and AMP by the luciferin-luciferase
reaction in fruits such as apples, pears and sweet cherries and some applications.
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